<?xml version="1.0" encoding="UTF-8"?> <!DOCTYPE article PUBLIC "-//NLM//DTD JATS (Z39.96) Journal Publishing DTD v1.2d1 20170631//EN" "JATS-journalpublishing1.dtd"> <ArticleSet> <Article> <Journal> <PublisherName>jmedicalcasereports</PublisherName> <JournalTitle>Frontiers in Medical Case Reports</JournalTitle> <PISSN>I</PISSN> <EISSN>S</EISSN> <Volume-Issue>Volume 1; Issue 5</Volume-Issue> <PartNumber/> <IssueTopic>Multidisciplinary</IssueTopic> <IssueLanguage>English</IssueLanguage> <Season>(Sep-Oct, 2020)</Season> <SpecialIssue>N</SpecialIssue> <SupplementaryIssue>N</SupplementaryIssue> <IssueOA>Y</IssueOA> <PubDate> <Year>-0001</Year> <Month>11</Month> <Day>30</Day> </PubDate> <ArticleType>Medical Case Reports</ArticleType> <ArticleTitle>Methods for The Detection of Carbapenemase in Clinical Microbiology</ArticleTitle> <SubTitle/> <ArticleLanguage>English</ArticleLanguage> <ArticleOA>Y</ArticleOA> <FirstPage>1</FirstPage> <LastPage>4</LastPage> <AuthorList> <Author> <FirstName>Leonor Aties</FirstName> <LastName>López</LastName> <AuthorLanguage>English</AuthorLanguage> <Affiliation/> <CorrespondingAuthor>N</CorrespondingAuthor> <ORCID/> <FirstName>Miracles of Charity Milá</FirstName> <LastName>Pascual</LastName> <AuthorLanguage>English</AuthorLanguage> <Affiliation/> <CorrespondingAuthor>Y</CorrespondingAuthor> <ORCID/> <FirstName>Gilberto Moya</FirstName> <LastName>Jústiz3</LastName> <AuthorLanguage>English</AuthorLanguage> <Affiliation/> <CorrespondingAuthor>Y</CorrespondingAuthor> <ORCID/> </Author> </AuthorList> <DOI/> <Abstract>Preliminary results obtained after applying two methods for the detection of carbapenemase, the colorimetric Blue-carba and the inactivating carbapenem, are presented, which were tested in six strains isolated from clinical samples, where satisfactory results were obtained after using both procedures. Taking into account the importance of these enzymes in Microbiology laboratories, due to the clinical-epidemiological impact they represent and the difficulty of not having molecular methods, these procedures are proposed for their detection.</Abstract> <AbstractLanguage>English</AbstractLanguage> <Keywords>Carbapenemases,Blue-carba methods and carbapenem inactivation,Clinical microbiology laboratories</Keywords> <URLs> <Abstract>https://jmedicalcasereports.org/ubijournal-v1copy/journals/abstract.php?article_id=8311&title=Methods for The Detection of Carbapenemase in Clinical Microbiology</Abstract> </URLs> <References> <ReferencesarticleTitle>References</ReferencesarticleTitle> <ReferencesfirstPage>16</ReferencesfirstPage> <ReferenceslastPage>19</ReferenceslastPage> <References>Daza Pand;eacute;rez RM. Bacterial resistance to antimicrobials: its importance in decision-making in daily practice. Therapeutic Information of the National Health System 1998; 22: 3. World Health Organization. Drug resistance. Importance of antimicrobial resistance for public health. Available at: http://www.who.int/drugresistance/AMR_Importance/es Slicing E. Detection of carbapenemase-producing Enterobacteriaceae in the laboratory routine. Rev Esp Quimioter 2015; 28: 8-11. Resurrecciand;oacute;n-Delgado C, Montenegro-Idrogo JJ, Chiappe-Gonzalez A, Vargas-Gonzales R, Cucho-Espinoza C, Mamani-Condori DH, Huaroto-Valdivia LM. Klebsiella pneumoniae New Delhi metalo-lactamase in a Peruvian national hospital. Peru Rev Peru med exp public health 2017; 1: 261-7. Khalifa HO, Soliman AM, Ahmed AM, Shimamoto T, Hara T, Ikeda M, Kuroo Y, Kayama S, Sugai M, Shimamoto T. High carbapenem resistance in clinical gram-negative pathogens isolated in Egypt. Microb Drug Resist 2017; 23: 838-844. Montand;uacute;far-Andrade FE, Mesa-Navas M, Aguilar-Londoand;ntilde;o C, Saldarriaga-Acevedo C, Quiroga-Echeverr A, Builes-Montaand;ntilde;o CE, Villa-Franco JP, Zuleta-Tobon JJ, Montand;uacute;far-Pantoja MC, Monsalve MA, Hernand;aacute;ndez C. Clinical experience with infections caused by carbapenemase-producing Klebsiella pneumoniae in a tertiary care teaching institution in Medellin, Colombia. Rev Infectio 2016; 20: 17-24. van der Zwaluw K, de Haan A, Pluister GN, Bootsma HJ, de Neeling AJ, Schouls LM. The carbapenem inactivation method (CIM), a simple and low-cost alternative for the Carba NP test to assess phenotypic carbapenemase activity in gram-negative rods. PloS one 2015; 10: e0123690. Molin C. Phenotypic Detection of Carbapenemases in Pseudomonas aeruginosa in Patients who attended the Hospital de Cland;iacute;nicas San Lorenzo from February to July 2013. Mem Inst Investig Science Health 2016; 14: 25-31. March Rosselland;oacute; GA. Rapid methods for the detection of bacterial resistance to antibiotics. Rev Enferm Infecc Microbiol Clin 2017; 35: 182-188. Mediavilla Gradolph C, Sand;aacute;inz Rodriguez R, Valverde Troya M, de Toro Peinado, Bermudez Ruand;iacute;z M P, Palop-Borrand;aacute;s B. Evaluation of an immunochromatographic assay for the detection of OXA-48 carbapenemase. Rev Esp Quimioter 2017; 30: 45-49.</References> </References> </Journal> </Article> </ArticleSet>