<?xml version="1.0" encoding="UTF-8"?> <!DOCTYPE article PUBLIC "-//NLM//DTD JATS (Z39.96) Journal Publishing DTD v1.2d1 20170631//EN" "JATS-journalpublishing1.dtd"> <ArticleSet> <Article> <Journal> <PublisherName>jmedicalcasereports</PublisherName> <JournalTitle>Frontiers in Medical Case Reports</JournalTitle> <PISSN>I</PISSN> <EISSN>S</EISSN> <Volume-Issue>Volume 1; Issue 3</Volume-Issue> <PartNumber/> <IssueTopic>Multidisciplinary</IssueTopic> <IssueLanguage>English</IssueLanguage> <Season>(May-Jun, 2020)</Season> <SpecialIssue>N</SpecialIssue> <SupplementaryIssue>N</SupplementaryIssue> <IssueOA>Y</IssueOA> <PubDate> <Year>-0001</Year> <Month>11</Month> <Day>30</Day> </PubDate> <ArticleType>Medical Case Reports</ArticleType> <ArticleTitle>Methylobacterium Radiotolerans Bacteremia in Hemodialysis Patients: The New Technique of Identification MALDI-TOF Mass Spectrometry</ArticleTitle> <SubTitle/> <ArticleLanguage>English</ArticleLanguage> <ArticleOA>Y</ArticleOA> <FirstPage>1</FirstPage> <LastPage>8</LastPage> <AuthorList> <Author> <FirstName>Noce</FirstName> <LastName>A</LastName> <AuthorLanguage>English</AuthorLanguage> <Affiliation/> <CorrespondingAuthor>N</CorrespondingAuthor> <ORCID/> <FirstName>Marrone</FirstName> <LastName>G</LastName> <AuthorLanguage>English</AuthorLanguage> <Affiliation/> <CorrespondingAuthor>Y</CorrespondingAuthor> <ORCID/> <FirstName>D’Agostini</FirstName> <LastName>C</LastName> <AuthorLanguage>English</AuthorLanguage> <Affiliation/> <CorrespondingAuthor>Y</CorrespondingAuthor> <ORCID/> <FirstName>Gambardella</FirstName> <LastName>G</LastName> <AuthorLanguage>English</AuthorLanguage> <Affiliation/> <CorrespondingAuthor>Y</CorrespondingAuthor> <ORCID/> <FirstName>Ottaviani</FirstName> <LastName>E</LastName> <AuthorLanguage>English</AuthorLanguage> <Affiliation/> <CorrespondingAuthor>Y</CorrespondingAuthor> <ORCID/> <FirstName>Fontana</FirstName> <LastName>C</LastName> <AuthorLanguage>English</AuthorLanguage> <Affiliation/> <CorrespondingAuthor>Y</CorrespondingAuthor> <ORCID/> <FirstName>Wilson Jones</FirstName> <LastName>G</LastName> <AuthorLanguage>English</AuthorLanguage> <Affiliation/> <CorrespondingAuthor>Y</CorrespondingAuthor> <ORCID/> <FirstName>Di Lauro</FirstName> <LastName>M</LastName> <AuthorLanguage>English</AuthorLanguage> <Affiliation/> <CorrespondingAuthor>Y</CorrespondingAuthor> <ORCID/> <FirstName>Ferrannini</FirstName> <LastName>M</LastName> <AuthorLanguage>English</AuthorLanguage> <Affiliation/> <CorrespondingAuthor>Y</CorrespondingAuthor> <ORCID/> <FirstName>Borboni</FirstName> <LastName>P</LastName> <AuthorLanguage>English</AuthorLanguage> <Affiliation/> <CorrespondingAuthor>Y</CorrespondingAuthor> <ORCID/> <FirstName>Di Daniele</FirstName> <LastName>N</LastName> <AuthorLanguage>English</AuthorLanguage> <Affiliation/> <CorrespondingAuthor>Y</CorrespondingAuthor> <ORCID/> </Author> </AuthorList> <DOI/> <Abstract>Central venous catheter (CVC) infections represent one of the most common complications in hemodialysis patients. Understanding the risk factors that induce CVC infection and identifying the microorganisms that cause them, is essential for developing preventive strategies. Therefore, it is important to develop prophylaxis and surveillance protocols, but also to determine adequate diagnostic criteria that include the use of new laboratory tools, useful in identifying a wider range of pathogenic microorganisms. We reported a clinical case of CVC related infection caused by the gram negative Methylobacterium radiotolerans in a hemodialysis patient. Matrix-assisted laser desorption/ionization-time of flight is a type of mass spectrometry (MALDI-TOF MS) which utilizes microbial protein mass distribution analysis, generating a signal that is decoded and represented as a peak on a Cartesian reference system. MALDI-TOF MS was fundamental in the identification of the pathogen, which remained previously unidentified using routine laboratory techniques. The use of MALDI-TOF MS has therefore allowed the pathogen’s tempestive identification, accurate diagnosis and targeted antibiotic treatment, positively impacting on infection resolution, prognosis and patient survival.</Abstract> <AbstractLanguage>English</AbstractLanguage> <Keywords>Infections,Hemodialysis,MALDI-TOF MS</Keywords> <URLs> <Abstract>https://jmedicalcasereports.org/ubijournal-v1copy/journals/abstract.php?article_id=7395&title=Methylobacterium Radiotolerans Bacteremia in Hemodialysis Patients: The New Technique of Identification MALDI-TOF Mass Spectrometry</Abstract> </URLs> <References> <ReferencesarticleTitle>References</ReferencesarticleTitle> <ReferencesfirstPage>16</ReferencesfirstPage> <ReferenceslastPage>19</ReferenceslastPage> <References>Allon M, Depner TA, Radeva M, Bailey J, Beddhu S, Butterly D, Coyne DW, Gassman JJ, Kaufman AM, Kaysen GA, Lewis JA, Schwab SJ, Group HS. Impact of dialysis dose and membrane on infection-related hospitalization and death: results of the HEMO Study. J Am Soc Nephrol 2003; 14: 1863-1870. Cordovana M, Deni A, Kostrzewa M, Abdalla M, Ambretti S. First report of Methylobacterium radiotolerans bacteraemia identified by MALDI-TOF mass spectrometry. New Microbes New Infect 2019; 30: 100546. de Cal M, Cazzavillan S, Cruz D, Nalesso F, Brendolan A, Rassu M, Ronco C. [Methylobacterium radiotolerans bacteremia in hemodialysis patients]. G Ital Nefrol 2009; 26: 616-620. Eleftheriadis T, Liakopoulos V, Leivaditis K, Antoniadi G, Stefanidis I. Infections in hemodialysis: a concise review - Part 1: bacteremia and respiratory infections. Hippokratia 2011; 15: 12-17. Ferrara P and Albano L. The adherence to guidelines for preventing CVC-related infections: a survey among Italian health-care workers. BMC Infect Dis 2018; 18: 606. Fisher M, Golestaneh L, Allon M, Abreo K, Mokrzycki MH. Prevention of Bloodstream Infections in Patients Undergoing Hemodialysis. Clin J Am Soc Nephrol 2020; 15: 132-151. Gahlot R, Nigam C, Kumar V, Yadav G, Anupurba S. Catheter-related bloodstream infections. Int J Crit Illn Inj Sci 2014; 4: 162-167. Golestaneh L and Mokrzycki MH. Prevention of hemodialysis catheter infections: Ointments, dressings, locks, and catheter hub devices. Hemodial Int 2018; 22: S75-S82. Jaber BL. Bacterial infections in hemodialysis patients: pathogenesis and prevention. Kidney Int 2005; 67: 2508-2519. Kaneko M, Tominaga Y, Sakamoto K, Shikata H. Hemodialysis vascular access infection caused by Methylobacterium radiotolerans: The first confirmed case in Japan. J Infect Chemother 2020; 26: 107-109. Karkar A. Infection control guidelines in hemodialysis facilities. Kidney Res Clin Pract 2018; 37: 1-3. Kato S, Chmielewski M, Honda H, Pecoits-Filho R, Matsuo S, Yuzawa Y, Tranaeus A, Stenvinkel P, Lindholm B. Aspects of immune dysfunction in end-stage renal disease. Clin J Am Soc Nephrol 2008; 3: 1526-1533. Kovaleva J, Degener JE, van der Mei HC. Methylobacterium and its role in health care-associated infection. J Clin Microbiol 2014; 52: 1317-1321. Lai CC, Cheng A, Liu WL, Tan CK, Huang YT, Chung KP, Lee MR, Hsueh PR. Infections caused by unusual Methylobacterium species. J Clin Microbiol 2011; 49: 3329-3331. Li L, Tarrand JJ, Han XY. Microbiological and clinical features of four cases of catheter-related infection by Methylobacterium radiotolerans. J Clin Microbiol 2015; 53: 1375-1379. McCann M and Moore ZE. Interventions for preventing infectious complications in haemodialysis patients with central venous catheters. Cochrane Database Syst Rev 2010; 20(1). Mermel LA, Allon M, Bouza E, Craven DE, Flynn P, Oand;#39;Grady NP, Raad, II, Rijnders BJ, Sherertz RJ, Warren DK. Clinical practice guidelines for the diagnosis and management of intravascular catheter-related infection: 2009 Update by the Infectious Diseases Society of America. Clin Infect Dis 2009; 49: 1-45. Nagorny SA, Aleshukina AV, Aleshukina IS, Ermakova LA, Pshenichnaya NY. The application of proteomic methods (MALDI-toff MS) for studying protein profiles of some nematodes (dirofilaria and ascaris) for differentiating species. Int J Infect Dis 2019; 82: 61-65. Noce A, Fabrini R, Dessi M, Bocedi A, Santini S, Rovella V, Pastore A, Tesauro M, Bernardini S, Di Daniele N, Ricci G. Erythrocyte glutathione transferase activity: a possible early biomarker for blood toxicity in uremic diabetic patients. Acta Diabetol 2014; 51: 219-224. Noce A, Rovella V, Marrone G, Cattani G, Zingaretti V, Limongi D, Dand;#39;Agostini C, Sorge R, Casasco M, Di Daniele N, Ricci G, Bocedi A. Hemodialysis biomarkers: total advanced glycation end products (AGEs) against oxidized human serum albumin (HSAox). Acta Diabetol 2019. Patel R. MALDI-TOF MS for the diagnosis of infectious diseases. Clin Chem 2015; 61: 100-111. Santoro D, Benedetto F, Mondello P, Pipito N, Barilla D, Spinelli F, Ricciardi CA, Cernaro V, Buemi M. Vascular access for hemodialysis: current perspectives. Int J Nephrol Renovasc Dis 2014; 7: 281-294. Sarnak MJ and Jaber BL. Mortality caused by sepsis in patients with end-stage renal disease compared with the general population. Kidney Int 2000; 58: 1758-1764. Schmidli J, Widmer MK, Basile C, de Donato G, Gallieni M, Gibbons CP, Haage P, Hamilton G, Hedin U, Kamper L, Lazarides MK, Lindsey B, Mestres G, Pegoraro M, Roy J, Setacci C, Shemesh D, Tordoir JHM, van Loon M, Esvs Guidelines C, Kolh P, de Borst GJ, Chakfe N, Debus S, Hinchliffe R, Kakkos S, Koncar I, Lindholt J, Naylor R, Vega de Ceniga M, Vermassen F, Verzini F, Esvs Guidelines R, Mohaupt M, Ricco JB, Roca-Tey R. Editorand;#39;s Choice - Vascular Access: 2018 Clinical Practice Guidelines of the European Society for Vascular Surgery (ESVS). Eur J Vasc Endovasc Surg 2018; 55: 757-818. Tazza L, Galli F, Mandolfo S, Forneris G, Di Dio M, Palumbo R, Gallieni M, Bonforte G, Carnabuci A, Cavatorta F, Aloisi M, Carbonari L, Investigators PSS. [Indications for vascular grafts as dialysis access; consensus from the Italian experience]. G Ital Nefrol 2010; 27: 512-521. Truant AL, Gulati R, Giger O, Satishchandran V, Caya JG. Methylobacterium bacteremia in AIDS. Clin Microbiol Infect 1998; 4: 112-113. Wang TY, Hung CY, Shie SS, Chou PC, Kuo CH, Chung FT, Lo YL, Lin SM. The clinical outcomes and predictive factors for in-hospital mortality in non-neutropenic patients with candidemia. Medicine (Baltimore) 2016; 95: e3834.</References> </References> </Journal> </Article> </ArticleSet>